Professional Summary

  • Dr. Pauletti is an Associate Professor of Biopharmaceutics & Pharmacokinetics at the James L. Winkle College of Pharmacy. His research interests focus on molecular pharmaceutics with an emphasis on membrane transport. Current activities include rational design, fabrication, and evaluation of innovative, non-viral nanocarriers as vectors for targeted drug delivery applications to improve therapeutic efficacy of small molecules, biotechnology drugs, and antitumor agents. Dr. Pauletti has published more than 100 scientific abstracts and papers. He is an inventor on 15 U.S. patents and patent applications protecting various drug delivery technologies and contributed to one GenBank entry. Dr. Pauletti served on more than 40 MS/PhD committees and is engaged in the education of professional PharmD and MS/PhD students enrolled in Pharmaceutical Sciences and Engineering graduate programs. As an advisor to pharmaceutical companies, Dr. Pauletti has facilitated preclinical and early clinical development of different molecular entities relevant to women's health and cancer therapy.

Education

  • MPharm - Pharmacy (1987): Swiss Federal Institute of Technology
  • PhD - Biopharmaceutics/Pharmacokinetics (1994): Swiss Federal Institute of Technology

Research/Clinical Interests

  • Research interests focus on molecular pharmaceutics with an emphasis on membrane transport. Current activities include rational design, fabrication, and evaluation of innovative, non-viral nanocarriers as vectors for targeted drug delivery applications to improve therapeutic efficacy of small molecules, biotechnology drugs, and antitumor agents. Specifically, we apply phage display technology to identify unique peptide ligands that facilitate selective intracellular delivery and/or transepithelial transport of colloidal nanocarriers such as nanoparticles, liposomes, and niosomes. In addition, we are engaged in collaborative research projects with UC faculty in Material Science and Internal Medicine aimed at developing multifunctional nanocomposite structures suitable for drug delivery and non-invasive imaging applications.

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Post Graduate Training/Education
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  • Postdoctoral Fellow - Peptide Drug Delivery (1993 - 1997)The University of Kansas, Pharmaceutical Chemistry Department (Dr. Ronald T. Borchardt)
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Research Grants
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  • UC Research Council. "Development of Nonviral DNA Delivery Systems for Placental Gene Therapy"
  • Bill & Melinda Gates Foundation. "Tampon-Like Foam Device for Dual-Purpose Contraception"
  • National Institute of General Medical Sciences. "In vitro Human Tissue Model for Intravaginal Drug Delivery"
  • R15 GM06763904/10/2003-03/31/2007. National Institute of General Medical Sciences. Vaginal Drug Delivery of P-Glycoprotein Substrates, PI, Closed.
  • HD04851203/01/2005-02/28/2007. National Institute of Child Health and Human Development. Peptide Ligands for Placental Transcytosis Receptors, PI, Closed.
  • PHRMA-02-01-A0-S0-E001/01/2000-12/31/2000. Pharmaceutical Research and Manufacturers of America. Rational Prodrug Design to Overcome Drug Resistance, PI, Closed.
  • 07/01/2002-06/30/2004. American Cancer Society - Ohio Chapter. Growth Inhibition of Metastatic Pancreatic Cancer in vivo, PI, Closed.
  • DAMD17-00-1-0202-03-A0-S0-E007/01/2000-06/30/2003. Department of the Army. Improved Delivery of Doxorubicin Into Drug-Resistant Breast Cancer Cells, PI, Closed.
  • OPP102525211/01/2010-04/30/2012. Bill & Melinda Gates Foundation. Tampon-like Foam Device for Dual-purpose Contraception, PI, Closed.
  • MAT / 1 R43 GM093563-0109/06/2010-03/05/2012. National Institute of General Medical Sciences. In Vitro Human Tissue Model for Intravaginal Delivery, PI, Closed.
  • Master Study Agreement10/01/2011-10/02/2016. Dow Corning Corporation. In Vitro Permeation Properties of Polymethylsiloxanes, PI, Active.
  • JS-2658 Fellowship - Allam09/24/2012-09/23/2013. Embassy of the Arab Republic of Egypt. Design and Evaluation of Medicated Polymeric Nanoparticles for Tumor Therapy, PI, Active.
  • JS-266109/24/2012-09/23/2013. Embassy of the Arab Republic of Egypt. Formulation and Delivery of Gene with Peptide-targeted Nanocarriers, PI, Active.
  • OPP108702205/01/2013-10/31/2014. Bill & Melinda Gates Foundation. Paper Based Diagnostics for Tropical Disease Detection, Collaborator, Active.
  • Noura Hassan Abdellah Ali, JS-266109/20/2013-09/19/2014. Embassy of the Arab Republic of Egypt. Formulation and Delivery of Gene With Peptide-targeted Nanocarriers, PI, Awarded.
  • Scholarship-Basma12/13/2013-12/31/2014. Embassy of the Arab Republic of Egypt. Solid Lipid Nanoparticle Formulation for Alendronate - Basma Nagy Abd El Hamid, PI, Awarded.
  • Scholarship-Nermin12/13/2013-12/31/2014. Embassy of the Arab Republic of Egypt. Therapeutic Efficacy of Oral Heparin - Scholar Nermin El Sayed El Eraky Ali , PI, Awarded.
  • URC Interdisciplinary Awards04/01/2014-03/31/2015. UC's University Research Council. Clinical Assessment of a Novel Paper-based Microfluidics Device: Towards Patient-Controlled Monitoring of Blood Coagulation, Collaborator, Active.
  • Scholarship-Ahmed01/01/2015-12/31/2015. Embassy of the Arab Republic of Egypt. "Tumor-targeting Chitosan Nanoparticles" - Scholar: Sheryhan Ahmed, PI, Awarded.
  • NSF IIP-150023604/01/2015-09/30/2016. National Science Foundation. Developing an Engineering Prototype for Ultra-Low-Cost Blood Coagulation Diagnostics Using Paper-Based Microfluidics, Collaborator, Awarded.
  • MatTek sub R44GM10816402/01/2016-01/31/2017. National Institute of General Medical Sciences. Validation of In an Vitro Human Small Intestine Model for Pharmaceutical Development, PI, Active.
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Peer Reviewed Publications (in chronological order)
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  • Dunn, A.W., Zhang, Y., Mast, D.B., Pauletti, G.M., Xu, H., Zhang, J., Ewing, R.C., and Shi, D. (2016). In-vitro Depth-dependent Hyperthermia of Human Mammary Gland Adenocarcinoma.. Mater. Sci. Eng. C, 69, 12-16. doi:10.1016/j.msec.2016.06.026
  • Tucker, G., DeSilva, B., Dressman, J., Ito, M., Kumamoto, T., Mager, D., Mahler, H.C., Maitland-van der Zee, A.H., Pauletti, G.M., Sasaki, H., Shah, V., Tang, D., and Ward, M. (2016). Current Challenges and Potential Opportunities for the Pharmaceutical Sciences to Make Global Impact: An FIP Perspective.. J. Pharm. Sci., 105 (9), 344-351. doi:10.1016/j.xphs.2015.12.001
  • Abd Ellah, N., Taylor, L., Ayres, N., Elmahdy, M.M., Fetih, G.N., Ibrahim, E.A., and Pauletti, G.M. (2016). NF-κB Decoy Polyplexes Decrease P-glycoprotein-mediated Multidrug Resistance in Colorectal Cancer Cells.. Cancer Gene Ther., 23 (5), 149-155. doi:10.1038/cgt.2016.17
  • Gad, S.F., Fetih, G.N., Tous, S.S., and Pauletti, G.M. (2016). Fabrication of Lipid-coated Chitosan Nanoparticles.. Br. J. Pharm. Res., 13, 1-8. doi:10.9734/BJPR/2016/28714
  • Dunn, A.W., Sadat, M.E., Mast, D.B., Pauletti, G.M., Xu, H., Zhang, J., Ewing, R.C., and Shi, D. (2015). Photothermal Effects and Toxicity of Fe3O4 Nanoparticles via Near Infrared Laser Irradiation for Cancer Therapy.. Mater. Sci. Eng. C, 46, 97-102. doi:10.1016/j.msec.2014.09.043
  • Abd Ellah, N., Taylor, L., Ayres, N., Pauletti, G.M., and Jones, H.N. (2015). Development of Non-viral, Trophoblast-specific Gene Delivery for Placental Therapy.. PLoS One, 10, e0140879. doi:10.1371/journal.pone.0140879
  • Eraky, N., Mohamed, D.F., Attia, M.A., and Pauletti, G.M. (2015). Hydrophobic Ion-Pairing of Low Molecular Weight Heparin with Cetyltrimethylammonium Bromide.. Int. J. Pharma Sci. Res., 6, 558-565.
  • Abd El Hamid, B., Soliman, G.M., Attia, M.A., and Pauletti, G.M. (2015). An Ion Pairing Approach to Enhance Oral Bioavailability of Alendronate.. J. Sci. Res. Reports, 5, 344-351. doi:10.9734/JSRR/2015/13975
  • Karkare, S., Chhipa, R., Liu, X., Henry, H., Gasilina, A., Nassar, N., Pauletti, G.M., Ghosh, P.K., and Dasgupta, B. (2014). Direct Inhibition of Retinoblastoma Phosphorylation by Nimbolide Causes Cell Cycle Arrest and Suppresses Glioblastoma Growth.. Clinical Cancer Res., 20 (1), 199-212. doi:10.1158/1078-0432.CCR-13-0762
  • Li, H., Han, D., Pauletti, G. M., & Steckl, A. J. (2014). Blood Coagulation Screening using a Paper-based Microfluidic Lateral Flow Device. Lab Chip, 14 (20), 4035-41. doi:10.1039/c4lc00716f
  • Abd Ellah, N., Potter, S.J., Taylor, L., Ayres, N., Elmahdy, M.M., Fetih, G.N., Ibrahim, E.A., and Pauletti, G.M. (2014). Safety and Efficacy of Amine-containing Methacrylate Polymers as Non-viral Gene Delivery Vectors.. J. Pharm. Tech. Drug Res., 3, 2. doi:10.7243/2050-120X-3-2
  • Abd Ellah, Taylor, L., Ayres, N., Elmahdy, M.M., Fetih, G.N., Ibrahim, E.A., and Pauletti, G.M. (2014). Increased Transfection Efficiency of Lipid-Coated Methacrylate-Based Gene Delivery Systems.. Int. J. Pharma Sci. Res., 5, 812-819.
  • Sadat, M.E., Kaveh-Baghbadorani, M., Dunn, A.W., Wagner, H., Ewing, R.C., A.W., Zhang, J., Xu, H., Pauletti, G.M., Mast, D.B., and Shi, D. (2014). Photoluminescence and the Effect of Structure in Fe3O4 Nanoparticles.. Appl. Phys. Lett., 105, 091903. doi:10.1063/1.4895133
  • Sadat, M.E., Patel, R., Sookoor, J., Budko, S., Ewing, R.C., Zhang, J., Xu, H., Wang, Y., Pauletti, G.M., Mast, D.B., and Shi, D. (2014). Effect of Spatial Confinement on Magnetic Hyperthermia via Dipolar Interactions in Fe3O4 Nanoparticles for Biomedical Applications.. Mater. Sci. Eng. C, 42, 52-63. doi:10.1016/j.msec.2014.04.064
  • Allam, A. A., Sadat, M. E., Potter, S. J., Mast, D. B., Mohamed, D. F., Habib, F. S., & Pauletti, G. M. (2013). Stability and magnetically induced heating behavior of lipid-coated Fe3O4 nanoparticles.. Nanoscale Research Letters, 8 (1), 426. doi:10.1186/1556-276X-8-426
  • Wang, F., Pauletti, G. M., Wang, J., Zhang, J., Ewing, R. C., Wang, Y., & Shi, D. (2013). Dual surface-functionalized Janus nanocomposites of polystyrene/Fe?O?@SiO? for simultaneous tumor cell targeting and stimulus-induced drug release.. Advanced Materials (Deerfield Beach, Fla.), 25 (25), 3485-9. doi:10.1002/adma.201301376
  • Cai, X., Dong, C., Dong, H., Wang, G., Pauletti, G. M., Pan, X., Wen, H., Mehl, I., Li, Y., & Shi, D. (2012). Effective gene delivery using stimulus-responsive catiomer designed with redox-sensitive disulfide and acid-labile imine linkers.. Biomacromolecules, 13 (4), 1024-34. doi:10.1021/bm2017355
  • Wen, H.Y., Dong, H.Q., Xie, W.J., Li, Y.Y., Wang, K., Pauletti, G.M., and Shi, D. (2011). Rapidly Disassembling Nanomicelles with Disulfide-linked PEG Shell for Glutathione-mediated Intracellular Drug Delivery. ChemComm., 47, 3550-3552. doi:10.1039/C0CC04983B
  • Basha, S., Vaidhyanathan, S., and Pauletti, G.M. (2011). Selection of Peptide Ligands for Human Placental Transcytosis Systems using in vitro Phage Display. Methods Mol. Biol., 716, 141-156. doi:10.1007/978-1-61779-012-6_8
  • Li, X., Wen, H., Dong, H., Xue, W., Pauletti, G. M., Cai, X., Xia, W., Shi, D., & Li, Y. (2011). Self-assembling nanomicelles of a novel camptothecin prodrug engineered with a redox-responsive release mechanism.. Chemical Communications (Cambridge, England), 47 (30), 8647-9. doi:10.1039/c1cc12495a
  • Wen, H., Dong, H., Xie, W., Li, Y., Wang, K., Pauletti, G. M., & Shi, D. (2011). Rapidly disassembling nanomicelles with disulfide-linked PEG shells for glutathione-mediated intracellular drug delivery.. Chemical Communications (Cambridge, England), 47 (12), 3550-2. doi:10.1039/c0cc04983b
  • Basha, S., Vaidhyanathan, S., & Pauletti, G. M. (2011). Selection of peptide ligands for human placental transcytosis systems using in vitro phage display.. Methods in Molecular Biology (Clifton, N.J.), 716, 141-56. doi:10.1007/978-1-61779-012-6_8
  • Cho, H., Dong, Z, Pauletti, G.M., Zhang, J., Xu, H., Gu, H., Wang, L., Ewing, R.C., Huth, Ch., Wang, F., and Shi, D. (2010). Fluorescent Superparamagnetic Nanospheres for Drug Storage, Targeting, and Imaging - A Multifunctional Nanocarrier System for Cancer Diagnosis and Treatment. ACS Nano, 4, 5389-5404. doi:10.1021/nn101000e
  • Cho, H., Dong, Z., Pauletti, G. M., Zhang, J., Xu, H., Gu, H., Wang, L., Ewing, R. C., Huth, C., Wang, F., & Shi, D. (2010). Fluorescent, superparamagnetic nanospheres for drug storage, targeting, and imaging: a multifunctional nanocarrier system for cancer diagnosis and treatment.. ACS Nano, 4 (9), 5398-404. doi:10.1021/nn101000e
  • Kawedia, J. D., Jiang, M., Kulkarni, A., Waechter, H. E., Matlin, K. S., Pauletti, G. M., & Menon, A. G. (2008). The protein kinase A pathway contributes to Hg2+-induced alterations in phosphorylation and subcellular distribution of occludin associated with increased tight junction permeability of salivary epithelial cell monolayers.. The Journal of Pharmacology and Experimental Therapeutics, 326 (3), 829-37. doi:10.1124/jpet.107.135798
  • Narang, V. S., Pauletti, G. M., Gout, P. W., Buckley, D. J., & Buckley, A. R. (2007). Sulfasalazine-induced reduction of glutathione levels in breast cancer cells: enhancement of growth-inhibitory activity of Doxorubicin.. Chemotherapy, 53 (3), 210-7. doi:10.1159/000100812
  • Dixit, S. G., Zingarelli, B., Buckley, D. J., Buckley, A. R., & Pauletti, G. M. (2005). Nitric oxide mediates increased P-glycoprotein activity in interferon-{gamma}-stimulated human intestinal cells.. American Journal of Physiology. Gastrointestinal and Liver Physiology, 288 (3), G533-40. doi:10.1152/ajpgi.00248.2004
  • D'Souza, V. M., Buckley, D. J., Buckley, A. R., & Pauletti, G. M. (2003). Extracellular glucose concentration alters functional activity of the intestinal oligopeptide transporter (PepT-1) in Caco-2 cells.. Journal of Pharmaceutical Sciences, 92 (3), 594-603. doi:10.1002/jps.10325
  • D'Souza, V. M., Shertzer, H. G., Menon, A. G., & Pauletti, G. M. (2003). High glucose concentration in isotonic media alters caco-2 cell permeability.. AAPS PharmSci, 5 (3), E24. doi:10.1208/ps050324
  • Plageman, L. R., Pauletti, G. M., & Skau, K. A. (2002). Characterization of acetylcholinesterase in Caco-2 cells.. Experimental Biology and Medicine (Maywood, N.J.), 227 (7), 480-6.
  • Gangwar, S., Pauletti, G. M., Siahaan, T. J., Stella, V. J., & Borchardt, R. T. (1999). Synthesis of an esterase-sensitive cyclic prodrug of a model hexapeptide having enhanced membrane permeability and enzymatic stability using an acyloxyalkoxy promoiety.. Methods in Molecular Medicine, 23, 37-51. doi:10.1385/0-89603-517-4:37
  • Gudmundsson, O. S., Pauletti, G. M., Wang, W., Shan, D., Zhang, H., Wang, B., & Borchardt, R. T. (1999). Coumarinic acid-based cyclic prodrugs of opioid peptides that exhibit metabolic stability to peptidases and excellent cellular permeability.. Pharmaceutical Research, 16 (1), 7-15.
  • Sudoh, M., Pauletti, G. M., Yao, W., Moser, W., Yokoyama, A., Pasternak, A., Sprengeler, P. A., Smith, A. B., Hirschmann, R., & Borchardt, R. T. (1998). Transport characteristics of peptidomimetics. Effect of the pyrrolinone bioisostere on transport across Caco-2 cell monolayers.. Pharmaceutical Research, 15 (5), 719-25.
  • T Borchardt R, Jeffrey, Siahaan, Gangwar, & Pauletti, (1997). Improvement of oral peptide bioavailability: Peptidomimetics and prodrug strategies.. Advanced Drug Delivery Reviews, 27 (2-3), 235-256.
  • Okumu, F. W., Pauletti, G. M., Vander Velde, D. G., Siahaan, T. J., & Borchardt, R. T. (1997). Effect of restricted conformational flexibility on the permeation of model hexapeptides across Caco-2 cell monolayers.. Pharmaceutical Research, 14 (2), 169-75.
  • Pauletti, G. M., Okumu, F. W., & Borchardt, R. T. (1997). Effect of size and charge on the passive diffusion of peptides across Caco-2 cell monolayers via the paracellular pathway.. Pharmaceutical Research, 14 (2), 164-8.
  • Pauletti, G. M., Gangwar, S., Wang, B., & Borchardt, R. T. (1997). Esterase-sensitive cyclic prodrugs of peptides: evaluation of a phenylpropionic acid promoiety in a model hexapeptide.. Pharmaceutical Research, 14 (1), 11-7.
  • Pauletti, G. M., Gangwar, S., Okumu, F. W., Siahaan, T. J., Stella, V. J., & Borchardt, R. T. (1996). Esterase-sensitive cyclic prodrugs of peptides: evaluation of an acyloxyalkoxy promoiety in a model hexapeptide.. Pharmaceutical Research, 13 (11), 1615-23.
  • Huth, Ch., Shi, D., Wang, F., Carrahar, D., Lian, J., Lu, F., Zhang, J., Ewing, R.C., and Pauletti, G.M.(In Press). Phospholipid Assembly on Superparamagnetic Nanoparticles for Thermoresponsive Drug Delivery Applications. NanoLIFE.
  • Narang, V. S., Pauletti, G. M., Gout, P. W., Buckley, D. J., & Buckley, A. R. Suppression of cystine uptake by sulfasalazine inhibits proliferation of human mammary carcinoma cells.. Anticancer Research, 23 (6C), 4571-9.
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Published Abstracts
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  • Eleraky, N.E., Swarnakar, N.K., Mohamed, D.F., Attia, M.A., and Pauletti, G.M. (2016). Permeation-enhancing Nanoparticle Formulation to Enable Oral Absorption of Enoxaparin.. 30th Annual Meeting of the AAPS, November 13 – 17, Denver, CO (25R0900).
  • Abd El-Hamid, B.N., Swarnakar, N.K., Soliman, G.M., Shafie, M.A., and Pauletti, G.M. (2016). Encapsulation of Alendronate-Polyethyleneimine Complexes into Nanostructured Lipid Carriers Enhances Intestinal Permeation in vitro.. 30th Annual Meeting of the AAPS,, November 13 – 17, Denver, CO (23T1000).
  • Potter, S.J., Domoradzki, J.Y., and Pauletti, G.M. (2015). Comparative in vitro Absorption Models to Estimate Dietary Assimilation of Pharmaceuticals in Fish.. 29th Annual Meeting of the AAPS,, October 25 – 29, Orlando, FL (T2006).
  • Eleraky, N.E., Mohamed, D.F., Attia, M.A., and Pauletti, G.M. (2015). Thermodynamic Analysis of the Interaction between Low Molecular Weight Heparin and Cetyltrimethylammonium Bromide.. 29th Annual Meeting of the AAPS,, October 25 – 29, Orlando, FL (T3109).
  • Abd El-Hamid, B.N., Soliman, G.M., Shafie, M.A., and Pauletti, G.M. (2015). Hydrophobic Ion Pairing to Augment Loading Efficiency of Alendronate in Nanostructured Lipid Carriers.. 29th Annual Meeting of the AAPS,, October 25 – 29, Orlando, FL (W4098).
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Book Chapter
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  • Vromans, H and Pauletti, G.M. (2015). Product DesignIn Y. Bouwman & P. Le Brun (Eds.) ,Practical Pharmaceutics(pp.347-356), ). Royal Dutch Pharmacists Association :Amsterdam.
  • Pauletti, G.M. (2009). Physicochemcial and Biological Factors Affecting in vivo Performance of DrugsIn W.A. Ritschel & G.L Kearns (Eds.) ,Handbook of Basic Pharmacokinetics(pp.67-80), ). American Pharmacists Association :Washington, DC.
  • Pauletti, G.M. (2009). Correlation of Clinical Response with Drug DispositionIn W.A. Ritschel & G.L Kearns (Eds.) ,Handbook of Basic Pharmacokinetics(pp.357-366), ). American Pharmacists Association :Washington, DC.
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Recent Awards/Honors
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  • Faculty Service Recognition Award.(UC Winkle College of Pharmacy),2015
  • Faculty Research Recognition Award.(UC Winkle College of Pharmacy),2015
  • Scientific Secretary (elected 2015-2019), International Pharmaceutical Federation (FIP),2015
  • Vice President, FIP Board of Pharmaceutical Sciences (elected, 2014 – 2015),2014
  • Faculty Service Recognition Award.(UC Winkle College of Pharmacy),2014
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Keywords
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  • Nanopharmaceutics, targeted drug delivery, membrane transport
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More Information
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  • Current research team members include: Shindu Thomas (PhD student), Dr. Ezhilarasi Perumal (Postdoctoral Fellow), and Dr. Shiva Raju (Postdoctoral Fellow)
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