Professional Summary

  • Dr. Risinger studies Hereditary Hemolytic Anemias (HHAs), including
    red blood cell (RBC) membrane disorders and Sickle Cell Disease
    (SCD), with particular emphasis on the role of proteins controlling
    RBC hydration. In addition to contributing to our understanding of
    the function of proteins that control cell hydration in normal RBCs
    and their pathological contribution in HHAs, these studies may
    provide information on genetic modifiers for SCD and help suggest
    additional therapeutic targets for treatment.

Education

  • BSN: Marycrest College, 1972
  • MA: University of Iowa, 1976
  • PhD: University of Iowa, 1983

Research/Clinical Interests

  • Research interests include:
    • Genomic Polymorphisms in Sickle Cell Disease
    • Genes that Affect Hydration and Reactive Oxygen Species (ROS) Production in
            Erythrocytes 
    • Erythrocyte Cytoskeleton Disorders
    • Erythrocyte Volume Regulation
     

Credentials

  • RN, Ohio License 386925
  • ELS, Editor in the Life Sciences

[Collapse All]

[Expand All]


Positions and Work Experience
Back to Top
  • 10-2013,Assistant Professor, Research Track,University of Cincinnati, College of Nursing,Cincinnati, OH
  • 03-2006-09-2012,Research Associate, Cancer and Blood Diseases Institute,Cincinnati Children's Hospital Medical Center,Cincinnati, OH
  • 09-2000-09-2005,Research Associate, Department of Molecular Genetics, Biochemistry and Microbiology,University of Cincinnati,Cincinnati, OH
  • 11-1999-06-2000,Independent Contractor, Department of Environmental Health,University of Cincinnati,Cincinnati, OH
  • 02-1998-08-1999,Associate Scientist, Beth Israel Deaconess Medical Center,Harvard University,Boston, MA
  • 05-1989-02-1998,Assistant Professor, Department of Medicine,Tufts University,Boston, MA
  • 02-1989-02-1998,Assistant Investigator, Department of Biomedical Research,St. Elizabeth's Medical Center,Boston, MA
  • 07-1987-01-1989,Research Associate, Department of Biophysics,Johns Hopkins University,Baltimore, MD
  • 07-1983-06-1987,Postdoctoral Fellow, Department of Biophysics,Johns Hopkins University,Baltimore, MD
  • 08-1979-08-1981,Teaching Assistant, Department of Anatomy and Cell Biology,University of Iowa,Iowa City, IA
  • 01-1976-05-1978,Instructor, Department of Nursing,Mt. Mercy University,Cedar Rapids, IA
  • 05-1972-08-1974,Staff Nurse,Mercy Hospital,Davenport, IA
Back to Top
Peer Reviewed Publications (in chronological order)
Back to Top
  • Niss, O., Chonat, S., Dagaonkar, N., Almansoori, M.O., Kerr, K., Rogers, Z.R., McGann, P.T., Quarmyne, M.-O., Risinger, M., Zhang, K., and Kalfa, T.A. (2016). Genotype-phenotype correlations in hereditary elliptocytosis and hereditary pyropoikilocytosis. . Blood Cells Mol. Dis..
  • Konstantinidis, D.G., Giger, K.M., Risinger, M., Pushkaran, S., Zhou, P., Dexheimer, P., Yermeni, S., Andreassen P., Klingmuller, U., Palis, J., Zheng Y., and Kalfa, T.A. (2015). Cytokinesis failure in RhoA-deficient mouse erythroblasts involves actomyosin and midbody dysregulation and triggers p53 activation.. Blood, 126, 1473-1482.
  • Pan, D., Kalfa, T.A., Wang, D., Risinger, M., Crable, S., Ottlinger, A., Chandra, S., Mount, D.B., Hubner, C.A., Franco, R.S., and Joiner, C.H. (2011). K-Cl cotransporter gene expression during human and murine erythroid differentiation. . J. Biol. Chem., 286, 30492-30503.
  • Quarmyne, M.O., Risinger, M., Linkugel, A., Frazier, A., and Joiner, C. (2011). Volume regulation and KCl cotransport in reticulocyte populations of sickle and normal red blood cells. . Blood Cells Mol. Dis., 47, 95-99.
  • Hammill, A.M., Risinger, M.A., Joiner, C.H., Keddache, M., and Kalfa, T.A. (2011). Compound heterozygosity for two novel mutations in the erythrocyte protein 4.2 gene causing spherocytosis in a Caucasian patient. . Br. J. Haematol., 152, 780-783.
  • Rinehart, J., Maksimova, Y.D., Tanis, J.E., Stone, K.L., Hodson, C.A., Zhang, J., Risinger, M., Pan, W., Wu, D., Colangelo, C.M., Forbush, B., Joiner, C.H., Gulcicek, E.E., Gallagher, P.G., and Lifton, R.P. (2009). Sites of regulated phosphorylation that control K-Cl cotransporter activity.. Cell, 138, 525-536.
  • Joiner, C.H., Rettig, R.K., Jiang, M., Risinger, M., and Franco, R.S. (2007). Urea stimulation of KCl cotransport induces abnornmal volume reduction in sickle reticulocytes. . Blood, 109, 1728-1735.
  • Goss, K.H., Risinger, M.A., Kordich, J.J., Sanz, M.M., Straughen, J.E., Sovek, L.E., Capobianco, A.J., German, J., Boivin, G.P., and Groden, J. (2002). Enhanced tumor formation in mice heterozygous for Blm mutation. . Science, 297, 2051-2053.
  • Oakley, G.G., Loberg, L.I., Yao, J., Risinger, M.A., Yunker, R.L., Zernik-Kobak, M., Khanna, K.K., Lavin, M.F., Carty, M.P., and Dixon, K. (2001). UV-induced hyperphosphorylation of replication protein A depends on DNA replication and expression of ATM protein.. Mol. Biol. Cell, 12, 1199-1213.
  • Sytkowski, A.J., Lunn, E.D., Risinger, M.A., and Davis, K.L. (1999). An erythropoietin fusion protein comprised of identical repeating domains exhibits enhanced biological properties.. J. Biol. Chem., 274, 24773-24778.
  • Risinger, M.A., Korsgren, C., and Cohen, C.M. (1996). Role of N-myristylation in targeting of band 4.2 (pallidin) in non-erythroid cells.. Exp. Cell Res., 229, 421-431.
  • Hayette, S., Morle, L., Bozon, M., Ghanem, E., Risinger, M., Korsgren, C., Tanner, M.J.A., Fattoum, S., Cohen, C.M., and Delaunay, J. (1995). A point mutation in the protein 4.2 gene (allele 4.2 Tozeur) associated with hereditary haemolytic anaemia. . Br. J. Haematol., 89, 762-770.
  • Risinger, M.A., Dotimas, E.M., and Cohen, C.M. (1992). Human erythrocyte band 4.2, a high copy number membrane protein, is N-myristylated.. J. Biol. Chem., 267, 5680-5685.
  • Zachary, J.M., Cleveland, G., Kwock, L., Lawrence, T., Weissman, R.M., Nabell, L., Fried, F.A., Staab, E.V., Risinger, M.A., and Lin, S. (1986). Actin filament organization of the Dunning R-3327 rat prostatic adenocarcinoma system: Correlation with metastatic potential. . Cancer Res., 46, 926-932.
  • Wilkins, J.A., Risinger, M.A., and Lin, S. (1986). Studies on proteins that co-purify with smooth muscle vinculin. Identification of immunologically related species in focal adhesions of nonmuscle and z-lines of muscle cells. . J. Cell Biol., 103, 1483-1494.
  • Risinger, M.A., and Larsen, W.J. (1982). Interaction of filipin with junctional membrane at different stages of the junction's life history. . Tissue and Cell, 15, 1-15.
  • Risinger, M.A., and Larsen, W.J. (1981). Endocytosis of cell-cell junctions and spontaneous cell disaggregation in a cultured human ovarian adenocarcinoma (COLO 316). . Tissue and Cell, 13, 413-430.
Back to Top
Published Abstracts
Back to Top
  • Gupta, S., Baskin, J., Kalfa, T., Risinger, M., and Zhang, K. (2016). Hereditary hemolytic anemia due to red cell monovalent cation leak in a patient with a novel band 3 mutation.. Pediatr. Blood Cancer, 63, S58-S59.
  • Risinger, M.A., Glogowska, E., Begtrup, A.H., Dagaonkar, N., Chonat, S., Joiner, C.H., Quinn, C.T., Kalfa, T.A., and Gallagher, P.G. (2014). The novel PIEZO1 mutation p.L2023V is causal for hereditary xerocytosis resulting in delayed channel inactivation and a dehydrated red blood cell phenotype.. Blood, 124, abstract 741.
  • Sokolic, R., Chonat, S., Risinger, M., Eckhaus, M., Garabedian, E., Calvo, K., Maric, I., Candotti, F., and Kalfa, T. (2014). Cation leak in red blood cells of patients with Wiskott-Aldrich Syndrome leads to non-immunologic hemolysis.. Blood, 124, abstract 1338.
  • Bakeer, N., Luo, H.-Y., Grimley, M.S., Risinger, M., Manganaris, P.T., Begtrup, A.H., Chui, D.H.K., and Kalfa, T.A. (2014). Hemoglobin Cincinnati: A novel beta globin gene mutation causing dominant beta thalassemia. . Pediatr. Blood Cancer, 61, S15.
  • Begtrup, A.H., Dagaonkar, N., Chonat, S., Giger, K.M., Husami, A., Kissell, D., Risinger, M., Joiner, C.H., Keddache, M., Zhang, K., and Kalfa, T.A. (2014). Development of a comprehensive rapid Next-Generation sequencing assay for the diagnosis of inherited hemolytic anemia. . Pediatr. Blood Cancer (61), S59.
  • Kalfa, T.A., Risinger M.A., Pushkaran, S., Dexheimer P., Husami, A., Kissell, D., Fletcher, D., Joiner, C.H., Keddache, M., and Zhang, K. (2012). Next generation sequencing for diagnostic testing of erythrocyte cytoskeleton disorders. . Blood, 120, abstract 976.
  • Rinehart, J., Maksimova, Y.D., Tanis, J.E., Stone, K.L., Zhang, J., Risinger, M., Pan, W., Wu, D., Colangelo, C.M., Forbush, B., Joiner, C.H., Gulcicek, E.E., Gallagher, P.G., and Lifton, R.P. (2009). Quantitative phosphoproteomics identifies sites in K-Cl cotransporters that regulate cell volume and neuronal excitation.. Http://www.asms.org., abstract 2246.
  • Risinger, M., Rinehart, J., Crable, S., Ottlinger, A., Winkelmann, R., Pan, D., Huebner, C., Gallagher, P.G., and Clinton H. Joiner. (2008). Structural and functional interactions of KCl Cotransport proteins KCC1 and KCC3 in sickle and normal erythrocyte membranes.. Blood, 112, abstract.
  • Pan, D., Kalfa, T.A., Wang, D., Risinger, M., Crable, S., Ciraolo, P., Franco, R.S., and Joiner, C.H. (2007). Change in expressional profile of KCl cotransporter genes during human erythroid differentiation.. Blood, 110, abstract 1709.
  • Feldman, L., Rouleau, C., and Risinger, M.A. (1998). Thrombopoietin has direct effects on the erythropoietin receptor in responsive erythroid progenitors.. Blood, 92, 580A.
  • Risinger, M.A., Derick, L.H., and Cohen, C.M. (1994). Melanocytes cultured from pallid mice are less melanized than C57 melanocytes and demonstrate differences in pallidin (band 4.2) localization. . Mol. Biol. Cell, 5, 366A.
  • Risinger, M.A., Korsgren, C., and Cohen, C.M. (1992). Expression and characterization of a nonmyristylated mutant human erythrocyte band 4.2. . Mol. Biol. Cell, 3, 211A.
  • Risinger, M.A., Dotimas, E.M., and Cohen, C.M. (1991). Both native and recombinant human erythrocyte band 4.2 produced in a eukaryotic expression system are myristylated.. J. Cell Biol., 115, 58A.
  • Risinger, M.A., and Cohen, C.M. (1990). Expression of human erythrocyte band 4.2 in a eukaryotic expression system. . J. Cell Biol..
  • Risinger, M.A., and Lin, S. (1989). Localization of tensin in chicken embryo fibroblasts during cell-substrate contact formation and disruption.. J. Cell Biol., 107, 256A.
  • Wilkins, J.A., Risinger, M.A., Coffey, E., and Lin, S. (1987). Purification of a vinculin binding protein from smooth muscle.. J. Cell Biol., 105, 130A.
  • Risinger, M.A., Wilkins, J.A., and Lin, S. (1987). Comparison of tensin and vinculin localizations in muscle and nonmuscle cells.. J. Cell Biol., 105, 130A.
  • Mullmann, T.J., Risinger, M.A., and Lin, S. (1987). Immunoblot analyses with a monoclonal antibody against capactin, a protein that caps the barbed end of F-actin. . J. Cell Biol., 105, 195A.
  • Lin, S., Risinger, M.A., Mullmann, T.J., Tseng, J., and Lin, D.C. (1987). Effects of neoplastic transformation on the activity and cellular localization of actin regulator proteins in nonmuscle cells. In: Proceedings of International Meeting on the Biology of an Ubiquitous Protein: Actin. Monza, Italy..
  • Wilkins, J.A., Risinger, M.A., and Lin, S. (1986). Immunologically related proteins at focal adhesions of nonmuscle and z-lines of muscle cells.. J. Cell Biol., 103, 111A.
  • Risinger, M.A., Zachary, J.M., Kwock, L., and Lin, S. (1984). Differences in cytoskeletal organization and endogenous cytochalasin-like activity between the low metastatic potential Dunning R-3327-H cell line and its high metastatic potential subline MAT LyLu.. J. Cell Biol., 99, 303A.
  • Larsen, W.J., and Risinger, M.A. (1982). The interaction of internalized cytoplasmic gap junction membrane with sterol-rich vesicles in SW-13 cells.. Anat. Rec., 202, 108A.
  • Risinger, M.A., and Larsen, W.J. (1981). Relationship of filipin-sterol complexes to tight junctions during their interiorization in a cultured ovarian adenocarcinoma.. J. Cell Biol., 91, 261A.
  • Risinger, M.A., and Larsen, W.J. (1981). Filament-membrane interactions in COLO 316 cells fixed prior to S-1 decoration. . Anat. Rec., 199, 212A.
  • Risinger, M.A., and Larsen, W.J. (1980). Endocytosis and degradation of tight junctions in the human ovarian adenocarcinoma cell line, COLO 316. . Anat. Rec., 196, 158A.
  • Larsen, W.J., Tung, H.N., Risinger, M.A., and Murray, S.A. (1979). Actin facilitated endocytosis and degradation of gap and tight junctions. . J. Cell Biol., 83, 87A.
Back to Top
Book Chapter
Back to Top
  • Risinger, M.A., and Groden, J. (2004). recQ DNA helicase family in genetic stability.In W.J. Lennarz and M.D. Lane (Eds.) ,Encyclopedia of Biological Chemistry, vol 3(pp.660-664). Oxford, UK :Elsevier Science.
  • Korsgren, C., Risinger, M.A. and Cohen, C.M. (2000). Human erythrocyte protein band 4.2. In A. Haeberli (Eds.) ,Human Protein DataWeinheim, Germany :VCH.
  • Lin, S., Risinger, M.A., and Butler, J.A. (1988). A model for protein-protein interactions involved in the linkage of the actin cytoskeleton to transmembrane receptors for extracellular matrix proteins.In P.M. Bayley (Eds.) ,Structure, Interactions and Assembly of Cytoskeletal and Extracellular Proteins :Springer Series in Biophysics.
  • Lin, S., Risinger, M.A., Wilkins, J.A., Mullmann, T.J., Butler, J.A., Wachsstock, D.H., and Lin, D.C. (1987). Proteins involved in the assembly and membrane-attachment of actin filaments in muscle and nonmuscle cells..
  • Lin, S., Lees, A., Risinger, M.A., Cribbs, D.H., Wilkins, J.A., and Lin, D. (1986). Effects of several actin binding proteins on nucleation, elongation and depolymerization of actin filaments in vitro.In H. Ishikawa, H. Sato, and S. Hatano (Eds.) ,Cell Motility: Mechanisms and RegulationTokyo, Japan :Univ. of Tokyo Press.
  • Larsen, W.J., and Risinger, M.A. (1985). The dynamic life histories of intercellular membrane junctions. In B.H. Satir (Eds.) ,Modern Cell Biology, vol 4 :Alan R. Liss, Inc..
Back to Top
Review
Back to Top
  • Risinger, M.A., and Groden, J. (2004). Crosslinks and crosstalk: Human cancer syndromes and DNA repair defects. ,Cancer Cell ,6:539-545.
Back to Top
Courses Taught
Back to Top
  • NURS 8022 ,Advanced Physiology and Pathophysiology. Graduate
Back to Top
Service
Back to Top
  • Departmental Service ,Faculty Search Committee ,Committee Member(2014-to Present).
  • Departmental Service ,PhD Program Preliminary Examination Committee ,Committee Member(2014-to Present).
Back to Top